secondary antibody (Vector Laboratories)

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Vector Laboratories secondary antibody
Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rabbit+igg/ImmPRESS+Universal+PLUS+Polymer+Kit%2C+Peroxidase+(Horse+Anti-Mouse%2FRabbit+IgG)/pmc13675904-65-20-28
Average 94 stars, based on 246 article reviews

secondary antibody - by Bioz Stars, 2026-10

94/100 stars

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Incubation:

Article Title: NF‑κB‑driven LUZP1 promotes metastasis and chemoresistance in head and neck squamous cell carcinoma.
Article Snippet: After blocking with 5% normal goat serum (cat. no. 005‐000‐121; Jackson ImmunoResearch Laboratories, Inc.) for 1 h at room temperature, slides were incubated over‐ night at 4 ̊C with anti‐LUZP1 primary antibody (1:15 dilution; cat. no. 17483‐1‐AP, Proteintech group Inc.). .. Following three PBS washes, sections were incubated with biotinylated goat anti‐rabbit Igg (1:400; cat. no. BA‐1000; vector Laboratories, Inc.) at room temperature for 1 h, and signal amplification was performed using the ABC‐hRP kit (cat. no. K‐6100; vector Laboratories, Inc.). visualization was achieved with gill's hematoxylin (cat. no. 3801520, Leica Biosystems) for nuclear counterstaining. .. Finally, slides were dehydrated through graded ethanol (cat. no. 459836; Merck KgAa; 75, 95 and 100%; 3 min each), cleared in xylene (cat. no. 4312, Muto Pure Chemicals Co. Ltd.) 10 min twice and mounted with coverslips.

Article Title: Alternations in the expression of hypoxia inducible factor 1 alpha (HIF1α) and selected angiogenesis-related genes in the hen ovary in relation to follicle development and atresia
Article Snippet: .. After washing with TBS, the sections were incubated for 90 min with DyLight 594-conjugated anti-rabbit IgG (#DI-1594-1.5; Vector Laboratories, Burlingame, CA, USA). .. The sections were then washed again in TBS and mounted with VECTASHIELD® VibranceTM Antifade Mounting Medium containing DAPI (Vector Laboratories, Burlingame, CA, USA).

Article Title: Methods for treating drug resistant cancers
Article Snippet: .. Sections were blocked then incubated overnight at 4° C. in primary antibody integrin αvβ3 (LM609) or β3 (Cell signaling), Glut3 (Santa Cruz Biotechnology), GFAP (cell signaling), βIIITubulin (Sigma-Aldrich), Nestin (Fisher Scientific), CD133 (Miltenyi Biotech) followed by biotin-conjugated anti-rabbit IgG and an avidin-biotin peroxidase detection system with 3,3′-diaminobenzidine substrate (Vector) then counterstained with hematoxylin. .. A Nikon Eclipse C1 Confocal microscope was used for imaging.

Amplification:

Article Title: N-terminally acetylated Met11-Tau: a new pathological truncated Tau species with functional relevance in Alzheimer's disease
Article Snippet: Brain sections were washed with PBS-Triton (0.2%), treated for 30 min with 0.3% H 2 O 2 and nonspecific binding was blocked with MOM (Mouse on Mouse) blocking reagent (Vector Laboratories, Burlingame, CA) or horse serum (1:100 in PBS; Vector Laboratories) for 1 h. The sections were then incubated with the primary antibody (Table S2) in PBS-Triton 0.2% overnight at 4°C. .. After 3 × 10 min washes, labeling was amplified using a biotinylated anti-mouse or anti-rabbit IgG (1:400 in PBS-Triton 0.2%; Vector Laboratories) for 1 h, followed by the ABC kit (1:400 in PBS; Vector Laboratories), and labeling was completed using 0.5 mg/mL DAB (Vector Laboratories) in 50 mmol/l Tris–HCl, pH 7.6, containing 0.075% H 2 O 2 . .. Brain sections were mounted on SuperFrost slides, dehydrated through a graded series of alcohol and toluene, and then mounted with Vectamount medium (#H5000; Vector Laboratories) and coverslips (#LCO2460M; Labelians) for microscopic analysis.

Labeling:

Avidin-Biotin Assay:

other:

Article Title: Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts
Article Snippet: Commercially available secondary antibodies used in the current study were as follows: Alexa Fluor 488‐conjugated anti‐mouse IgG (Thermo Fisher Scientific, A11001 [RRID: AB_2534069]); Alexa Fluor 568‐conjugated anti‐rabbit IgG (Thermo Fisher Scientific, A11036 [RRID: AB_10563566]); HRP‐conjugated anti‐rabbit IgG (Vector Laboratories, PI‐1000 [RRID: AB_2336198]).

Chromatin Immunoprecipitation:

Article Title: KDM3A catalyses the oxidation of acetyl-lysine to hydroxyacetyl-lysine on histone H3K9.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used actin (Sigma, A1978, Lot: 065M4837V), Flag (Sigma, F1804, Lot: SLBN5629V), Flag (Sigma, F7425, Lot: 085M4774V), HA (sc-7392, Lot: G1818), HA (CST, 3724S, Lot: 8), HIF-1α (BD Biosciences, 610959, Lot: 5296905), H3K9ac (Abcam, ab4441, GR3290365-1, GR3229436-1, GR3253211), H3K9acOH fraction: R63, P1, F1.3 & F1.4, R43, P1, and F1.4 in this study), H3K9me3 (Abcam, ab8898, Lot: GR3217826-1, GR3245584-1), H3K9me2 (Abcam, ab1220, Lot: GR325223-3, GR3228498-2, GR325223-4, GR3308902-5), H3K9me1 (EpiCypher, 13-0014, Lot: 14247001), H3K9me0 (Active Motif, 61399, Lot: 34612001), Histone H4 (Abcam, ab177840, Lot: GR3189348-8), KDM3A (Proteintech, 12835-1-AP, Lot: 00009716), KDM3B (CST, 2621S, Lot: 1), Vinculin (sigma, V9131, Lot: N/A), anti-Rabbit IgG (Vector, PI-1000-1) and anti-Mouse IgG (Vector, PI-2000-1). ..

Flow Cytometry:

Magnetic Resonance Imaging:

Saline:

Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
Article Snippet: .. Sections were then washed thoroughly in Tris-buffered saline (TBS) before applying horse radish peroxidase-conjugated ImmPRESS anti-mouse IgG (Vector Laboratories, MP-7402) or anti-rabbit IgG (Vector Laboratories, MP-7401) secondary antibodies for 30 min at RT. .. Antibody detection was visualised by incubating the sections for 10 min at RT with 3, 3′-diaminobenzidine (DAB; ImmPACT Vector Laboratories, SK-4105).

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